Kano Pillars have been sent crashing from the Federation Cup by Prime FC, who won 2-1 to advance to the round of 16.
Another lower league side, Crown FC beat Niger Tornadoes 5-4 on penalties, after both teams were deadlocked at 1-1 at the end of regulation time.
However, other Nigeria Premier League sides advanced to the next stage of the cup competition.
Sunshine Stars outscored Abia Comets 4-2 on penalties after full time scores stood at 1-1, Nasarawa stopped Go Round FC 1-0, Rivers United thumped Kaduna United 4-0 and Shooting Stars went past Almar FC 3-0.
Other results are ABS FC versus Enugu Rangers (abandoned, due to heavy downpour; Kogi United 1, Wikki Tourists 2; Warri Wolves 3 Jigawa Stars 0 (walk-over) as well as, FC Ifeanyiubah 1 Lobi Stars 0